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Article

A highly specific SpCas9 variant is identified by in vivo screening in yeast.

Nature biotechnology - 1 Mar 2018

Casini Antonio, Olivieri Michele, Petris Gianluca, Montagna Claudia, Reginato Giordano, Maule Giulia, Lorenzin Francesca, Prandi Davide, Romanel Alessandro, Demichelis Francesca, Inga Alberto, Cereseto Anna

Abstract excerpt

Despite the utility of CRISPR-Cas9 nucleases for genome editing, the potential for off-target activity limits their application, especially for therapeutic purposes. We developed a yeast-based assay to identify optimized Streptococcus pyogenes Cas9 (SpCas9) variants that enables simultaneous evaluation of on- and off-target activity. We screened a library of SpCas9 variants carrying random mutations in the REC3...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 29431739
DOI: 10.1038/nbt.4066
PMCID: PMC6066108

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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