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Article

Differential efficacies of Cas nucleases on microsatellites involved in human disorders and associated off-target mutations.

Nucleic acids research - 20 Aug 2021

Poggi Lucie, Emmenegger Lisa, Descorps-Declère Stéphane, Dumas Bruno, Richard Guy-Franck

Abstract excerpt

Microsatellite expansions are the cause of >20 neurological or developmental human disorders. Shortening expanded repeats using specific DNA endonucleases may be envisioned as a gene editing approach. Here, we measured the efficacy of several CRISPR-Cas nucleases to induce recombination within disease-related microsatellites, in Saccharomyces cerevisiae. Broad variations in nuclease performances were detected on...

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Identifiers

PMID: 34233005
DOI: 10.1093/nar/gkab569
PMCID: PMC8373144

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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