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Article

SMOOT libraries and phage-induced directed evolution of Cas9 to engineer reduced off-target activity.

PloS one - 1 Jan 2020

Cerchione Derek, Loveluck Katherine, Tillotson Eric L, Harbinski Fred, DaSilva Jen, Kelley Chase P, Keston-Smith Elise, Fernandez Cecilia A, Myer Vic E, Jayaram Hariharan, Steinberg Barrett E

Abstract excerpt

RNA-guided endonucleases such as Cas9 provide efficient on-target genome editing in cells but may also cleave at off-target loci throughout the genome. Engineered variants of Streptococcus pyogenes Cas9 (SpCas9) have been developed to globally reduce off-target activity, but individual off-targets may remain, or on-target activity may be compromised. In order to evolve against activity at specific off-targets...

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Share to Bacteriophage M13
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32298334
DOI: 10.1371/journal.pone.0231716
PMCID: PMC7161989

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