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Article

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.

Genome biology - 6 Oct 2017

Kulcsár Péter István, Tálas András, Huszár Krisztina, Ligeti Zoltán, Tóth Eszter, Weinhardt Nóra, Fodor Elfrieda, Welker Ervin

Abstract excerpt

BACKGROUND: The propensity for off-target activity of Streptococcus pyogenes Cas9 (SpCas9) has been considerably decreased by rationally engineered variants with increased fidelity (eSpCas9; SpCas9-HF1). However, a subset of targets still generate considerable off-target effects. To deal specifically with these targets, we generated new "Highly enhanced Fidelity" nuclease variants (HeFSpCas9s) containing...

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Share to Bacterial Proteins
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

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Identifiers

PMID: 28985763
DOI: 10.1186/s13059-017-1318-8
PMCID: PMC6389135

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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