Article

Directed evolution of CRISPR-Cas9 to increase its specificity

2017-12-19

Abstract excerpt

The use of CRISPR-Cas9 as a therapeutic reagent is hampered by its off-target effects. Although rationally designed S. pyogenes Cas9 (SpCas9) variants that display higher specificities than the wild-type SpCas9 protein are available, these attenuated Cas9 variants are often poorly efficient in human cells. Here, we have used a directed evolution approach in E. coli to obtain Sniper-Cas9, which shows high specifici...

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Literature Corpus work: 416b86f5-5826-5e02-b637-9cf5e3ea9610
DOI: 10.1101/237040

Open publication

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