Article
A Type II-B Cas9 nuclease with minimized off-targets and reduced chromosomal translocations in vivo.
Nature communications - 6 Sept 2023
Bestas Burcu, Wimberger Sandra, Degtev Dmitrii, Madsen Alexandra, Rottner Antje K, Karlsson Fredrik, Naumenko Sergey, Callahan Megan, Touza Julia Liz, Francescatto Margherita, Möller Carl Ivar, Badertscher Lukas, Li Songyuan, Cerboni Silvia, Selfjord Niklas, Ericson Elke, Gordon Euan, Firth Mike, Chylinski Krzysztof, Taheri-Ghahfarokhi Amir, Bohlooly-Y Mohammad, Snowden Mike, Pangalos Menelaos, Nuttall Barrett, Akcakaya Pinar, Sienski Grzegorz, Maresca Marcello
Abstract excerpt
Streptococcus pyogenes Cas9 (SpCas9) and derived enzymes are widely used as genome editors, but their promiscuous nuclease activity often induces undesired mutations and chromosomal rearrangements. Several strategies for mapping off-target effects have emerged, but they suffer from limited sensitivity. To increase the detection sensitivity, we develop an off-target assessment workflow that uses Duplex Sequencing....
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