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Article

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.

Nature - 28 Jan 2016

Kleinstiver Benjamin P, Pattanayak Vikram, Prew Michelle S, Tsai Shengdar Q, Nguyen Nhu T, Zheng Zongli, Joung J Keith

Abstract excerpt

CRISPR-Cas9 nucleases are widely used for genome editing but can induce unwanted off-target mutations. Existing strategies for reducing genome-wide off-target effects of the widely used Streptococcus pyogenes Cas9 (SpCas9) are imperfect, possessing only partial or unproven efficacies and other limitations that constrain their use. Here we describe SpCas9-HF1, a high-fidelity variant harbouring alterations...

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Source

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Identifiers

PMID: 26735016
DOI: 10.1038/nature16526
PMCID: PMC4851738

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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