Article
High-throughput creation and functional profiling of DNA sequence variant libraries using CRISPR-Cas9 in yeast.
Nature biotechnology - 1 Jul 2018
Guo Xiaoge, Chavez Alejandro, Tung Angela, Chan Yingleong, Kaas Christian, Yin Yi, Cecchi Ryan, Garnier Santiago Lopez, Kelsic Eric D, Schubert Max, DiCarlo James E, Collins James J, Church George M
Abstract excerpt
Construction and characterization of large genetic variant libraries is essential for understanding genome function, but remains challenging. Here, we introduce a Cas9-based approach for generating pools of mutants with defined genetic alterations (deletions, substitutions, and insertions) with an efficiency of 80-100% in yeast, along with methods for tracking their fitness en masse. We demonstrate the utility of...
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