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Article

High-fidelity SaCas9 identified by directional screening in human cells.

PLoS biology - 1 Jul 2020

Xie Haihua, Ge Xianglian, Yang Fayu, Wang Bang, Li Shuang, Duan Jinzhi, Lv Xiujuan, Cheng Congsheng, Song Zongming, Liu Changbao, Zhao Junzhao, Zhang Yu, Wu Jinyu, Gao Caixia, Zhang Jinwei, Gu Feng

Abstract excerpt

CRISPR-Staphylococcus aureus Cas9 (CRISPR-SaCas9) has been harnessed as an effective in vivo genome-editing tool to manipulate genomes. However, off-target effects remain a major bottleneck that precludes safe and reliable applications in genome editing. Here, we characterize the off-target effects of wild-type (WT) SaCas9 at single-nucleotide (single-nt) resolution and describe a directional screening system to...

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PMID: 32644995
DOI: 10.1371/journal.pbio.3000747
PMCID: PMC7347106

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