Article
Multiplex conditional mutagenesis in zebrafish using the CRISPR/Cas system.
Methods in cell biology - 1 Jan 2016
Yin L, Maddison L A, Chen W
Abstract excerpt
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system is a powerful tool for genome editing in numerous organisms. However, the system is typically used for gene editing throughout the entire organism. Tissue and temporal specific mutagenesis is often desirable to determine gene function in a specific stage or tissue and to bypass undesired consequences of...
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