Article
Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system.
Proceedings of the National Academy of Sciences of the United States of America - 20 Aug 2013
Jao Li-En, Wente Susan R, Chen Wenbiao
Abstract excerpt
A simple and robust method for targeted mutagenesis in zebrafish has long been sought. Previous methods generate monoallelic mutations in the germ line of F0 animals, usually delaying homozygosity for the mutation to the F2 generation. Generation of robust biallelic mutations in the F0 would allow for phenotypic analysis directly in injected animals. Recently the type II prokaryotic clustered regularly...
Topics
Join the communities discussing this publication.