Article

An optimized CRISPR/Cas toolbox for efficient germline and somatic genome engineering in <i>Drosophila</i>

2014-03-24

Abstract excerpt

The type II CRISPR/Cas system has recently emerged as a powerful method to manipulate the genomes of various organisms. Here, we report a novel toolbox for high efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evaluation reveals Cas9 lines with ubiquitous or germline restricted patterns of activity. We also...

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Literature Corpus work: dd79b3d5-636c-5ed9-beac-f9cca5273213
DOI: 10.1101/003541

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