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Article

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America - 22 Jul 2014

Port Fillip, Chen Hui-Min, Lee Tzumin, Bullock Simon L

Abstract excerpt

The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has emerged recently as a powerful method to manipulate the genomes of various organisms. Here, we report a toolbox for high-efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evaluation reveals Cas9...

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Identifiers

PMID: 25002478
DOI: 10.1073/pnas.1405500111
PMCID: PMC4115528

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