Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.

doi:10.1073/pnas.1405500111

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Article

Optimized CRISPR/Cas tools for efficient germline and somatic genome engineering in Drosophila.

Proceedings of the National Academy of Sciences of the United States of America - 22 Jul 2014

Port Fillip, Chen Hui-Min, Lee Tzumin, Bullock Simon L

Abstract excerpt

The type II clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated (Cas) system has emerged recently as a powerful method to manipulate the genomes of various organisms. Here, we report a toolbox for high-efficiency genome engineering of Drosophila melanogaster consisting of transgenic Cas9 lines and versatile guide RNA (gRNA) expression plasmids. Systematic evaluation reveals Cas9...

Topics

Alleles
Animals
Animals, Genetically Modified
Base Sequence
CRISPR-Cas Systems
DNA Repair
Drosophila melanogaster
Gene Targeting
Genes, Essential
Genes, Insect
Genetic Engineering

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genetic Vectors

Genome, Insect

Germ Cells

Molecular Sequence Data

Mutation

Phenotype

RNA

Source

Open in PubMed Open full text

Identifiers

PMID: 25002478
DOI: 10.1073/pnas.1405500111
PMCID: PMC4115528

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-25002478-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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