Article
Efficient genome editing in zebrafish using a CRISPR-Cas system.
Nature biotechnology - 1 Mar 2013
Hwang Woong Y, Fu Yanfang, Reyon Deepak, Maeder Morgan L, Tsai Shengdar Q, Sander Jeffry D, Peterson Randall T, Yeh J-R Joanna, Joung J Keith
Abstract excerpt
In bacteria, foreign nucleic acids are silenced by clustered, regularly interspaced, short palindromic repeats (CRISPR)--CRISPR-associated (Cas) systems. Bacterial type II CRISPR systems have been adapted to create guide RNAs that direct site-specific DNA cleavage by the Cas9 endonuclease in cultured cells. Here we show that the CRISPR-Cas system functions in vivo to induce targeted genetic modifications in...
Topics
- Animals
- Base Sequence
- CRISPR-Cas Systems
- DNA
- DNA Cleavage
- Embryo, Nonmammalian
- Endonucleases
- Genetic Engineering
- Genome
- Inverted Repeat Sequences
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- RNA Editing