Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.

doi:10.1007/978-1-4939-2917-7_16

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Article

Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Yin Linlin, Jao Li-En, Chen Wenbiao

Abstract excerpt

Several strategies have been developed to generate targeted gene disruptions in zebrafish.Here we developed a simple targeted gene inactivation strategy in zebrafish using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system. By injecting two...

Topics

Animals
Animals, Genetically Modified
CRISPR-Cas Systems
Gene Targeting
Mutagenesis, Site-Directed
Mutation
RNA Editing
RNA, Guide, CRISPR-Cas Systems
RNA, Messenger
Zebrafish

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