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Article

Generation of Targeted Mutations in Zebrafish Using the CRISPR/Cas System.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2015

Yin Linlin, Jao Li-En, Chen Wenbiao

Abstract excerpt

Several strategies have been developed to generate targeted gene disruptions in zebrafish.Here we developed a simple targeted gene inactivation strategy in zebrafish using a clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (Cas) system. By injecting two simple in vitro-synthesized components [Cas9 mRNA and single guide (sgRNA)] into one-cell-stage embryos, mutations of...

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Identifiers

PMID: 26285757
DOI: 10.1007/978-1-4939-2917-7_16

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