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Article

Multiplex Conditional Mutagenesis Using Transgenic Expression of Cas9 and sgRNAs.

Genetics - 1 Jun 2015

Yin Linlin, Maddison Lisette A, Li Mingyu, Kara Nergis, LaFave Matthew C, Varshney Gaurav K, Burgess Shawn M, Patton James G, Chen Wenbiao

Abstract excerpt

Determining the mechanism of gene function is greatly enhanced using conditional mutagenesis. However, generating engineered conditional alleles is inefficient and has only been widely used in mice. Importantly, multiplex conditional mutagenesis requires extensive breeding. Here we demonstrate a system for one-generation multiplex conditional mutagenesis in zebrafish (Danio rerio) using transgenic expression of...

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Identifiers

PMID: 25855067
DOI: 10.1534/genetics.115.176917
PMCID: PMC4492370

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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