Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes.

doi:10.1242/dev.134809

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Article

Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes.

Development (Cambridge, England) - 1 Jun 2016

Burger Alexa, Lindsay Helen, Felker Anastasia, Hess Christopher, Anders Carolin, Chiavacci Elena, Zaugg Jonas, Weber Lukas M, Catena Raul, Jinek Martin, Robinson Mark D, Mosimann Christian

Abstract excerpt

CRISPR-Cas9 enables efficient sequence-specific mutagenesis for creating somatic or germline mutants of model organisms. Key constraints in vivo remain the expression and delivery of active Cas9-sgRNA ribonucleoprotein complexes (RNPs) with minimal toxicity, variable mutagenesis efficiencies depe...

Topics

Alleles
Animals
Base Sequence
Binding Sites
CRISPR-Cas Systems
Embryo, Nonmammalian
Fluorescence
Genes, Reporter
Green Fluorescent Proteins
Morpholinos
Multiprotein Complexes

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutagenesis

Mutation

Phenotype

RNA, Guide, CRISPR-Cas Systems

Recombinant Fusion Proteins

Recombination, Genetic

Ribonucleoproteins

Source

Open in PubMed

Identifiers

PMID: 27130213
DOI: 10.1242/dev.134809

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-27130213-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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