Article
Insertional Mutagenesis by CRISPR/Cas9 Ribonucleoprotein Gene Editing in Cells Targeted for Point Mutation Repair Directed by Short Single-Stranded DNA Oligonucleotides.
PloS one - 1 Jan 2017
Rivera-Torres Natalia, Banas Kelly, Bialk Pawel, Bloh Kevin M, Kmiec Eric B
Abstract excerpt
CRISPR/Cas9 and single-stranded DNA oligonucleotides (ssODNs) have been used to direct the repair of a single base mutation in human genes. Here, we examine a method designed to increase the precision of RNA guided genome editing in human cells by utilizing a CRISPR/Cas9 ribonucleoprotein (RNP) complex to initiate DNA cleavage. The RNP is assembled in vitro and induces a double stranded break at a specific site...
Topics
- Alleles
- Base Sequence
- CRISPR-Cas Systems
- Clone Cells
- DNA Repair
- DNA, Single-Stranded
- Flow Cytometry
- Gene Editing
- Green Fluorescent Proteins
- HCT116 Cells
- Humans