Article
Acute multi-sgRNA knockdown of KEOPS complex genes reproduces the microcephaly phenotype of the stable knockout zebrafish model.
PloS one - 1 Jan 2018
Jobst-Schwan Tilman, Schmidt Johanna Magdalena, Schneider Ronen, Hoogstraten Charlotte A, Ullmann Jeremy F P, Schapiro David, Majmundar Amar J, Kolb Amy, Eddy Kaitlyn, Shril Shirlee, Braun Daniela A, Poduri Annapurna, Hildebrandt Friedhelm
Abstract excerpt
Until recently, morpholino oligonucleotides have been widely employed in zebrafish as an acute and efficient loss-of-function assay. However, off-target effects and reproducibility issues when compared to stable knockout lines have compromised their further use. Here we employed an acute CRISPR/Cas approach using multiple single guide RNAs targeting simultaneously different positions in two exemplar genes (osgep...
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