Article
Message amplification phenotyping (MAPPing): a technique to simultaneously measure multiple mRNAs from small numbers of cells.
BioTechniques - 1 Jan 2000
Brenner C A, Tam A W, Nelson P A, Engleman E G, Suzuki N, Fry K E, Larrick J W
Abstract excerpt
A rapid and highly sensitive technique (MAPPing: message amplification phenotyping) has been developed to simultaneously analyze the array of messenger RNAs made by small numbers of cells. The technique incorporates a micro-procedure for isolating RNA, reverse transcription of total cellular RNA to produce cDNA, and enzymatic amplification of cytokine-specific DNA fragments using the polymerase chain reaction. In...
Topics
- Base Sequence
- Biological Factors
- Biotechnology
- Cell Line
- Cytokines
- DNA
- Genetic Techniques
- Humans
- Kinetics
- Molecular Sequence Data
- Phenotype