Article
Clonal analysis of gene expression by PCR.
BioTechniques - 1 Sept 1990
Witsell A L, Schook L B
Abstract excerpt
A method for determining the heterogeneity of gene expression from isolated macrophage colonies is described. Clones derived using specific growth factors were isolated by soft agar cloning and used as sources of mRNA for analysis. Gene expression was monitored by the reverse transcription polymerase chain reaction method to permit a number of specific genes to be simultaneously amplified. The technique described...
Topics
- Animals
- Base Sequence
- Biotechnology
- Clone Cells
- DNA
- Gene Expression
- Growth Substances
- Macrophages
- Molecular Sequence Data
- Phenotype
- Polymerase Chain Reaction
- RNA