Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells.

doi:10.1093/nar/18.6.1587

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells.

Nucleic acids research - 25 Mar 1990

Timblin C, Battey J, Kuehl W M

Abstract excerpt

We describe a simple method for preparing a renewable source of subtractive cDNA which can be used as a hybridization probe or as insert which can be cloned into a variety of convenient vectors. This has been done by ligating a double-stranded oligonucleotide to each end of double-stranded subtractive cDNA, and then using this oligonucleotide sequence to amplify the heterogeneous population of cDNA molecules...

Topics

Animals
Base Sequence
Blotting, Northern
Cell Line
Cloning, Molecular
DNA, Neoplasm
Gene Expression
Genes, Immunoglobulin
Genetic Variation
Mice
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Nucleic Acid Amplification Techniques

Plasmacytoma

Polymerase Chain Reaction

RNA, Messenger

RNA, Neoplasm

Tumor Cells, Cultured

Source

Open in PubMed Open full text

Identifiers

PMID: 2326198
DOI: 10.1093/nar/18.6.1587
PMCID: PMC330530

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2326198-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Application for PCR technology to subtractive cDNA cloning: identification of genes expressed specifically in murine plasmacytoma cells.PMID 2326198Read ↗

A murine cDNA encodes a pan-epithelial glycoprotein that is also expressed on plasma cellsSemantic proximity Read ↗

A gene expression screen.Semantic proximity Read ↗

RaSH, a rapid subtraction hybridization approach for identifying and cloning differentially expressed genesSemantic proximity Read ↗

The selective isolation of novel cDNAs encoded by the regions surrounding the human interleukin 4 and 5 genesSemantic proximity Read ↗

Rapid isolation of immunoglobulin variable genes from cell lysates of rat hybridomas by polymerase chain reaction.Semantic proximity Read ↗

A computer-based method of selecting clones for a full-length cDNA project: simultaneous collection of negligibly redundant and variant cDNAs.Semantic proximity Read ↗

Cloning of full-length cDNA sequence of a novel candidate gene related to mouse cleft palate.Semantic proximity Read ↗

A method to identify cDNAs based on localization of green fluorescent protein fusion products.Semantic proximity Read ↗

Identification by subtractive hybridization of a spectrum of novel and unexpected genes associated with in vitro differentiation of human cytotrophoblast cellsSemantic proximity Read ↗

Isolation of novel complementary DNA clones from T lymphoma cells: one encodes a putative multiple membrane-spanning protein.Semantic proximity Read ↗

PCR amplification of mRNA directly from a crude cell lysate prepared by thermophilic protease digestion.Semantic proximity Read ↗

Isolation of a novel macrophage-specific gene by differential cDNA analysisSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.