Article
mRNA phenotyping for studying gene expression in small numbers of cells: platelet-derived growth factor and other growth factors in wound-derived macrophages.
American journal of respiratory cell and molecular biology - 1 Jan 1990
Rappolee D A, Werb Z
Abstract excerpt
The understanding of biologic and pathophysiologic processes in lung is aided by a technique for ascertaining the transcriptional phenotype of small numbers of cells examined in vivo. The mRNA phenotyping procedure consists of scaled-down methods for isolating mRNA from small numbers of cells, followed by reverse transcription of the RNA and specifically primed amplification of the cDNA by the polymerase chain...
Topics
- Animals
- Cell Line
- Epithelium
- Fluorescent Antibody Technique
- Gene Expression
- Growth Substances
- Immunoblotting
- Immunohistochemistry
- Lung Diseases
- Macrophages
- Mice