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Article

Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Gilliland G, Perrin S, Blanchard K, Bunn H F

Abstract excerpt

The expression of two cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3), has been investigated in MLA-144 cells before and after induction with phorbol 12-myristate 13-acetate. We describe an adaptation of the polymerase chain reaction (PCR) for highly accurate quantitation of mRNA or DNA from a small number of cells. Aliquots of the PCR mixture containing cDNA copies...

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Identifiers

PMID: 2181447
DOI: 10.1073/pnas.87.7.2725
PMCID: PMC53763

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