Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

doi:10.1073/pnas.87.7.2725

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Article

Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction.

Proceedings of the National Academy of Sciences of the United States of America - 1 Apr 1990

Gilliland G, Perrin S, Blanchard K, Bunn H F

Abstract excerpt

The expression of two cytokines, granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin 3 (IL-3), has been investigated in MLA-144 cells before and after induction with phorbol 12-myristate 13-acetate. We describe an adaptation of the polymerase chain reaction (PCR) for highly accurate quantitation of mRNA or DNA from a small number of cells. Aliquots of the PCR mixture containing cDNA copies...

Topics

Base Sequence
Cell Line
Colony-Stimulating Factors
DNA
DNA Probes
Gene Expression
Granulocyte-Macrophage Colony-Stimulating Factor
Growth Substances
Humans
Interleukin-3
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Nucleic Acid Amplification Techniques

Plasmids

Polymerase Chain Reaction

RNA, Messenger

Restriction Mapping

Tetradecanoylphorbol Acetate

Source

Open in PubMed Open full text

Identifiers

PMID: 2181447
DOI: 10.1073/pnas.87.7.2725
PMCID: PMC53763

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2181447-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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