Article
Evaluation of Secreted Cytokines by Multiplex Bead-Based Assay (X MAP Technology, Luminex).
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Lombardelli Letizia, Logiodice Federica, Kullolli Ornela, Piccinni Marie-Pierre
Abstract excerpt
The Luminex XMAP technology permits the simultaneous evaluation of numerous cytokines in several types of biological fluids (plasma, serum, liquor, follicular fluids, etc.) and in cell supernatants. Thus, multiplexing allows to achieve a time/cost economy and ensures that all the measurements are performed in the same conditions. Simultaneous measurement of cytokines with a multiplex bead-based assay has some...
Topics
- Animals
- Antibodies, Monoclonal
- Biotinylation
- Cells, Cultured
- Cytokines
- Fluorescent Antibody Technique
- Humans
- Phenotype
- Research Design
- Secretory Pathway
- T-Lymphocyte Subsets
