Article
Single cell analysis of cytokine gene coexpression during CD4+ T-cell phenotype development.
Proceedings of the National Academy of Sciences of the United States of America - 1 Aug 1995
Bucy R P, Karr L, Huang G Q, Li J, Carter D, Honjo K, Lemons J A, Murphy K M, Weaver C T
Abstract excerpt
CD4+ T cells from alpha beta-T-cell receptor transgenic mice were analyzed for coexpression of cytokine mRNAs during phenotype development using a double-label in situ hybridization technique. T cells that produced cytokines in the primary response were a fraction of the activated population, and only a minority of the cytokine-positive cells coexpressed two cytokines. In secondary responses, frequencies of...
Topics
- Animals
- CD4-Positive T-Lymphocytes
- Cell Differentiation
- Clone Cells
- Cytokines
- Female
- Gene Expression
- Lymphocyte Activation
- Mice
- Mice, Inbred BALB C
- Mice, Transgenic