Article
Assessment of lymphokine profiles in activated lymphocytes by semiquantitative PCR.
Journal of immunological methods - 18 Jun 1993
Kramnik I, Skamene E, Radzioch D
Abstract excerpt
Several methods are currently used to detect the expression of specific mRNAs in leukocytes. While Northern blot analysis and RNase protection assays are commonly chosen for quantitative assessment of mRNA levels, these methods require a significant quantity of RNA, making their use unfeasible wh...
Topics
- Animals
- Antibodies, Monoclonal
- Base Sequence
- Electrophoresis, Polyacrylamide Gel
- Gene Expression
- Lymphocyte Activation
- Lymphokines
- Mice
- Mice, Inbred BALB C
- Molecular Sequence Data
- Mycobacterium bovis
- Oligonucleotides
- Oligonucleotides, Antisense
- Phenotype
- Plasmids
- Polymerase Chain Reaction
- RNA
- RNA, Messenger