Quantitative determination of mRNA phenotypes by the polymerase chain reaction.

doi:10.1016/0003-2697(91)90122-a

Back to search

Article

Quantitative determination of mRNA phenotypes by the polymerase chain reaction.

Analytical biochemistry - 1 Jul 1991

Ballagi-Pordány A, Ballagi-Pordány A, Funa K

Abstract excerpt

A method for quantitative determination of specific cellular mRNA is described. The mRNA in a dilution series of total RNA was reverse transcribed by an oligo-dT primer and the cDNA was amplified by the polymerase chain reaction (PCR) using sets of specific primers. A 32P- or biotin-labeled speci...

Topics

Animals
Base Sequence
Cell Line
Cricetinae
DNA
Humans
Molecular Sequence Data
Nucleic Acid Hybridization
Phenotype
Polymerase Chain Reaction
RNA, Messenger
Regression Analysis

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.