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Article

Quantitative determination of mRNA phenotypes by the polymerase chain reaction.

Analytical biochemistry - 1 Jul 1991

Ballagi-Pordány A, Ballagi-Pordány A, Funa K

Abstract excerpt

A method for quantitative determination of specific cellular mRNA is described. The mRNA in a dilution series of total RNA was reverse transcribed by an oligo-dT primer and the cDNA was amplified by the polymerase chain reaction (PCR) using sets of specific primers. A 32P- or biotin-labeled specific probe was hybridized to the PCR products immobilized on nitrocellulose membrane. The intensity of the hybridization...

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PMID: 1888041
DOI: 10.1016/0003-2697(91)90122-a

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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