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Article

Single Crossover-Mediated Markerless Genome Engineering in Clostridium acetobutylicum.

Journal of microbiology and biotechnology - 28 Apr 2016

Lee Sang-Hyun, Kim Hyun Ju, Shin Yong-An, Kim Kyoung Heon, Lee Sang Jun

Abstract excerpt

A novel genome-engineering tool in Clostridium acetobutylicum was developed based on singlecrossover homologous recombination. A small-sized non-replicable plasmid, pHKO1, was designed for efficient integration into the C. acetobutylicum genome. The integrated pHKO1 plasmid backbone, which included an antibiotic resistance gene, can be excised in vivo by Flp recombinase, leaving a single flippase recognition...

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Identifiers

PMID: 26767573
DOI: 10.4014/jmb.1512.12012

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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