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A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas.

Applied biochemistry and biotechnology - 1 Aug 2024

Han Feng, Zhang Xiaoya, Chen Yunfei, Zhao Haixia, Wu Jieer, Yu Yongxin, Wang Yongjie

Abstract excerpt

Gene knockout is a widely used technique for engineering bacterial genomes, investigating the roles of genes in metabolism, and conferring biological characteristics. Herein, we developed a rapid, efficient, and simple method for the knockout of long gene cassettes in Pseudomonas spp., based on a traditional allelic exchange strategy. The upstream and downstream sequences of the target gene cluster to be deleted...

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PMID: 38103122
DOI: 10.1007/s12010-023-04806-1

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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