Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria.

BioTechniques - 1 Nov 2002

Marx Christopher J, Lidstrom Mary E

Abstract excerpt

Complete genome sequences are now available for many bacterial species that lack sophisticated genetic tools. We describe the development of a broad-host-range cre-lox system that allows antibiotic marker recycling in a variety of gram-negative bacteria. This system consists of an allelic exchange vector bearing a kanamycin cassette flanked by loxP sites and a tetracycline-resistant IncP plasmid that provides...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Burkholderia
Share to Genetic Vectors
Share to Gram-Negative Bacteria

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Integrases

Share to Kanamycin Resistance

Share to Substrate Specificity

Share to Tetracycline Resistance

Share to Viral Proteins

Source

Identifiers

PMID: 12449384
DOI: 10.2144/02335rr01

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Broad-host-range cre-lox system for antibiotic marker recycling in gram-negative bacteria.PMID 12449384Read ↗

Lox'd in translation: contradictions in the nomenclature surrounding common lox-site mutants and their implications in experiments.Semantic proximity Read ↗

Dup-In and DIRex: Techniques for Single-Step, Scar-Free Mutagenesis with Marker Recycling.Semantic proximity Read ↗

Constructing unmarked gene deletions in Legionella pneumophila.Semantic proximity Read ↗

Efficient dual-negative selection for bacterial genome editing.Semantic proximity Read ↗

Recombineering in Streptococcus mutans Using Direct Repeat-Mediated Cloning-Independent Markerless Mutagenesis (DR-CIMM).Semantic proximity Read ↗

CRISPR/Cas9 recombineering-mediated deep mutational scanning of essential genes in Escherichia coli.Semantic proximity Read ↗

Random mutagenesis using a mutator strain.Semantic proximity Read ↗

Recombineering: using drug cassettes to knock out genes in vivo.Semantic proximity Read ↗

Genome engineering in actinomycetes using site-specific recombinases.Semantic proximity Read ↗

An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia.Semantic proximity Read ↗

Alanine racemase mutants of Burkholderia pseudomallei and Burkholderia mallei and use of alanine racemase as a non-antibiotic-based selectable marker.Semantic proximity Read ↗

Targeted gene deletion in Candida albicans wild-type strains by MPAR flipping.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.