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Article

High-Frequency Targeted Mutagenesis in Pseudomonas stutzeri Using a Vector-Free Allele-Exchange Protocol.

Journal of microbiology and biotechnology - 28 Feb 2017

Gomaa Ahmed E, Deng Zhiping, Yang Zhimin, Shang Liguo, Zhan Yuhua, Lu Wei, Lin Min, Yan Yongliang

Abstract excerpt

The complexity of the bacterial recombination system is a barrier for the construction of bacterial mutants for the further functional investigation of specific genes. Several protocols have been developed to inactivate genes from the genus Pseudomonas. Those protocols are complicated and time-consuming and mostly do not enable easy construction of multiple knock-ins/outs. The current study describes a single and...

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Identifiers

PMID: 27817190
DOI: 10.4014/jmb.1608.08019

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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