Article
A rapid and simple method for constructing stable mutants of Acinetobacter baumannii.
BMC microbiology - 9 Nov 2010
Aranda Jesús, Poza Margarita, Pardo Belén G, Rumbo Soraya, Rumbo Carlos, Parreira José R, Rodríguez-Velo Patricia, Bou Germán
Abstract excerpt
BACKGROUND: Acinetobacter baumannii is a multidrug-resistant bacterium responsible for nosocomial infections in hospitals worldwide. Study of mutant phenotypes is fundamental for understanding gene function. The methodologies developed to inactivate A. baumannii genes are complicated and time-consuming; sometimes result in unstable mutants, and do not enable construction of double (or more) gene knockout mutant...
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