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Article

New vector for efficient allelic replacement in naturally nontransformable, low-GC-content, gram-positive bacteria.

Applied and environmental microbiology - 1 Nov 2004

Arnaud Maryvonne, Chastanet Arnaud, Débarbouillé Michel

Abstract excerpt

A shuttle vector designated pMAD was constructed for quickly generating gene inactivation mutants in naturally nontransformable gram-positive bacteria. This vector allows, on X-Gal (5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside) plates, a quick colorimetric blue-white discrimination of bacteria which have lost the plasmid, greatly facilitating clone identification during mutagenesis. The plasmid was used in...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 15528558
DOI: 10.1128/AEM.70.11.6887-6891.2004
PMCID: PMC525206

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Source receipt: pubmed, retrieved 7 Sept 2026.

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