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Article

I-SceI-mediated scarless gene modification via allelic exchange in Clostridium.

Journal of microbiological methods - 1 Jan 2015

Zhang Ning, Shao Lijun, Jiang Yu, Gu Yang, Li Qi, Liu Jinle, Jiang Weihong, Yang Sheng

Abstract excerpt

Although gene disruption in Clostridium spp. with the TargeTron technology is much more effective than single-crossover integration, it cannot achieve gene modification via allelic exchange. Here, we developed a targeted, nonpolar, scarless gene modification system based on the I-SceI endonuclease. First, a replicative plasmid containing homology arms on either side of the target sequence and I-SceI recognition...

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Identifiers

PMID: 25451462
DOI: 10.1016/j.mimet.2014.11.004

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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