Article
Establishment of a markerless mutation delivery system in Bacillus subtilis stimulated by a double-strand break in the chromosome.
PloS one - 1 Jan 2013
Shi Ting, Wang Guanglu, Wang Zhiwen, Fu Jing, Chen Tao, Zhao Xueming
Abstract excerpt
Bacillus subtilis has been a model for gram-positive bacteria and it has long been exploited for industrial and biotechnological applications. However, the availability of facile genetic tools for physiological analysis has generally lagged substantially behind traditional genetic models such as Escherichia coli and Saccharomyces cerevisiae. In this work, we have developed an efficient, precise and scarless...
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