Article
Targeted allele replacement mutagenesis of Corynebacterium pseudotuberculosis.
Applied and environmental microbiology - 1 May 2011
Walker Caray A, Donachie Willie, Smith David G E, Fontaine Michael C
Abstract excerpt
A two-step allele replacement mutagenesis procedure, using a conditionally replicating plasmid, was developed to allow the creation of targeted, marker-free mutations in Corynebacterium pseudotuberculosis. The relationship between homologous sequence length and recombination frequency was determined, and enhanced plasmid excision was observed due to the rolling-circle replication of the mutagenesis vector....
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