Article
Counterselection employing mutated pheS for markerless genetic deletion in Bacteroides species.
Anaerobe - 1 Dec 2016
Kino Yasuhiro, Nakayama-Imaohji Haruyuki, Fujita Masashi, Tada Ayano, Yoneda Saori, Murakami Kazuya, Hashimoto Masahito, Hayashi Tetsuya, Okazaki Katsuichiro, Kuwahara Tomomi
Abstract excerpt
Markerless gene deletion is necessary for multiple gene disruptions due to the limited number of antibiotic resistant markers for some bacteria. However, even in transformable strains, obtaining the expected mutation without a marker requires laborious screening of a large number of colonies. Previous studies had success in various bacteria with a counter-selection system where a conditional lethal gene was...
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