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Article

Knockout of a highly GC-rich gene in Burkholderia pyrrocinia by recombineering with freeze-thawing transformation.

Molecular plant pathology - 1 Jul 2021

Chen Feifei, Ye Jianren, Liu Wanhui, Chio Chonlong, Wang Wendy, Qin Wensheng

Abstract excerpt

Genetic transformation is a valuable and essential method that provides powerful insights into the gene function of microorganisms and contributes to the construction of engineered bacteria. Here, we developed a novel genetic transformation system to easily knock out a highly GC-rich gene (74.71% GC) from Burkholderia pyrrocinia JK-SH007, a biocontrol strain of poplar canker disease. This system revealed a...

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Identifiers

PMID: 33942460
DOI: 10.1111/mpp.13058
PMCID: PMC8232026

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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