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Article

Multiplex Genome Editing in Escherichia coli.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Jensen Sheila Ingemann, Nielsen Alex Toftgaard

Abstract excerpt

Lambda Red recombineering is an easy and efficient method for generating genetic modifications in Escherichia coli. For gene deletions, lambda Red recombineering is combined with the use of selectable markers, which are removed through the action of, e.g., flippase (Flp) recombinase. This PCR-based engineering method has also been applied to a number of other bacteria. In this chapter, we describe a recently...

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Identifiers

PMID: 29170956
DOI: 10.1007/978-1-4939-7295-1_8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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