Article
Tetramerization and interdomain flexibility of the replication initiation controller YabA enables simultaneous binding to multiple partners.
Nucleic acids research - 8 Jan 2016
Felicori Liza, Jameson Katie H, Roblin Pierre, Fogg Mark J, Garcia-Garcia Transito, Ventroux Magali, Cherrier Mickaël V, Bazin Alexandre, Noirot Philippe, Wilkinson Anthony J, Molina Franck, Terradot Laurent, Noirot-Gros Marie-Françoise
Abstract excerpt
YabA negatively regulates initiation of DNA replication in low-GC Gram-positive bacteria. The protein exerts its control through interactions with the initiator protein DnaA and the sliding clamp DnaN. Here, we combined X-ray crystallography, X-ray scattering (SAXS), modeling and biophysical approaches, with in vivo experimental data to gain insight into YabA function. The crystal structure of the N-terminal...
Topics
- Amino Acid Motifs
- Amino Acid Sequence
- Bacillus subtilis
- Bacterial Proteins
- Binding Sites
- DNA Replication
- DNA-Binding Proteins
- Intracellular Space
- Models, Molecular