Article
A nucleotide-dependent oligomerization of the Escherichia coli replication initiator DnaA requires residue His136 for remodeling of the chromosomal origin.
Nucleic acids research - 10 Jan 2020
Saxena Rahul, Stanley Christopher B, Kumar Pankaj, Cuneo Matthew J, Patil Digvijay, Jha Jyoti, Weiss Kevin L, Chattoraj Dhruba K, Crooke Elliott
Abstract excerpt
Escherichia coli replication initiator protein DnaA binds ATP with high affinity but the amount of ATP required to initiate replication greatly exceeds the amount required for binding. Previously, we showed that ATP-DnaA, not ADP-DnaA, undergoes a conformational change at the higher nucleotide concentration, which allows DnaA oligomerization at the replication origin but the association state remains unclear....
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.