Article
Effect of Multiple Clock Gene Ablations on the Circadian Period Length and Temperature Compensation in Mammalian Cells.
Journal of biological rhythms - 1 Feb 2016
Tsuchiya Yoshiki, Umemura Yasuhiro, Minami Yoichi, Koike Nobuya, Hosokawa Toshihiro, Hara Masayuki, Ito Hiroshi, Inokawa Hitoshi, Yagita Kazuhiro
Abstract excerpt
Most organisms have cell-autonomous circadian clocks to coordinate their activity and physiology according to 24-h environmental changes. Despite recent progress in circadian studies, it is not fully understood how the period length and the robustness of mammalian circadian rhythms are determined. In this study, we established a series of mouse embryonic stem cell (ESC) lines with single or multiplex clock gene...
Topics
- Animals
- CRISPR-Cas Systems
- Cell Line
- Circadian Clocks
- Circadian Rhythm
- Mice
- Mouse Embryonic Stem Cells
- Mutation
- Period Circadian Proteins
- Temperature