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Article

Conformational toggling controls target site choice for the heteromeric transposase element Tn7.

Nucleic acids research - 15 Dec 2015

Shi Qiaojuan, Straus Marco R, Caron Jeremy J, Wang Huasheng, Chung Yu Seon, Guarné Alba, Peters Joseph E

Abstract excerpt

The bacterial transposon Tn7 facilitates horizontal transfer by directing transposition into actively replicating DNA with the element-encoded protein TnsE. Structural analysis of the C-terminal domain of wild-type TnsE identified a novel protein fold including a central V-shaped loop that toggles between two distinct conformations. The structure of a robust TnsE gain-of-activity variant has this loop locked in a...

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Identifiers

PMID: 26384427
DOI: 10.1093/nar/gkv913
PMCID: PMC4678854

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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