Article
Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation.
The EMBO journal - 3 Jul 2000
Lu F, Craig N L
Abstract excerpt
Tn7 transposition has been hypothesized to require a heteromeric transposase formed by two Tn7-encoded proteins, TnsA and TnsB, and accessory proteins that activate the transposase when they are associated with an appropriate target DNA. This study investigates the mechanism of Tn7 transposase activation by isolation and analysis of transposase gain-of-function mutants that are active in the absence of these...
Topics
- Enzyme Activation
- Models, Molecular
- Mutation
- Protein Conformation
- Transposases