Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Tn5 transposase active site mutants.

The Journal of biological chemistry - 17 May 2002

Naumann Todd A, Reznikoff William S

Abstract excerpt

Tn5 transposase (Tnp) is a 53.3-kDa protein that is encoded by and facilitates movement of transposon Tn5. Tnp monomers contain a single active site that is responsible for catalyzing a series of four DNA breaking/joining reactions at one transposon end. Based on primary sequence homology and protein structural information, we designed and constructed a series of plasmids that encode for Tnps containing active...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Arginine
Share to Aspartic Acid
Share to Catalysis

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Glutamic Acid

Share to Lysine

Share to Molecular Weight

Share to Transposases

Share to Tyrosine

Source

Identifiers

PMID: 11877443
DOI: 10.1074/jbc.M200742200

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Tn5 transposase active site mutants.PMID 11877443Read ↗

A bifunctional DNA binding region in Tn5 transposase.Semantic proximity Read ↗

Three-dimensional structure of the Tn5 synaptic complex transposition intermediate.Semantic proximity Read ↗

Tn5 transposase loops DNA in the absence of Tn5 transposon end sequences.Semantic proximity Read ↗

Characterization of a Tn5 pre-cleavage synaptic complex.Semantic proximity Read ↗

Defining functional regions of the IS903 transposase.Semantic proximity Read ↗

Mutational analysis of the base flipping event found in Tn5 transposition.Semantic proximity Read ↗

DNA sequence bias during Tn5 transposition.Semantic proximity Read ↗

Pulling apart catalytically active Tn5 synaptic complexes using magnetic tweezers.Semantic proximity Read ↗

Structure-based recasting of a mammalian DNA transpososome as an obligate heterodimerSemantic proximity Read ↗

Tn5 synaptic complex formation: role of transposase residue W450.Semantic proximity Read ↗

Unlocking Tn3-family transposase activity in vitro unveils an asymetric pathway for transposome assembly.Semantic proximity Read ↗

Transposase subunit architecture and its relationship to genome size and the rate of transposition in prokaryotes and eukaryotes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.