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Article

Sleeping Beauty transposase structure allows rational design of hyperactive variants for genetic engineering.

Nature communications - 30 Mar 2016

Voigt Franka, Wiedemann Lisa, Zuliani Cecilia, Querques Irma, Sebe Attila, Mátés Lajos, Izsvák Zsuzsanna, Ivics Zoltán, Barabas Orsolya

Abstract excerpt

Sleeping Beauty (SB) is a prominent Tc1/mariner superfamily DNA transposon that provides a popular genome engineering tool in a broad range of organisms. It is mobilized by a transposase enzyme that catalyses DNA cleavage and integration at short specific sequences at the transposon ends. To facilitate SB's applications, here we determine the crystal structure of the transposase catalytic domain and use it to...

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Identifiers

PMID: 27025571
DOI: 10.1038/ncomms11126
PMCID: PMC4820933

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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