Article
Analysis of gain-of-function mutants of an ATP-dependent regulator of Tn7 transposition.
Journal of molecular biology - 19 Jan 2001
Stellwagen A E, Craig N L
Abstract excerpt
The bacterial transposon Tn7 is distinguished by its unusual discrimination among targets, being particularly attracted to certain target DNA and actively avoiding other DNA. Tn7 transposition is mediated by the interaction of two alternative transposon-encoded target selection proteins, TnsD and TnsE, with a common core transposition machinery composed of the transposase (TnsAB) and an ATP-dependent DNA-binding...
Topics
- Adenosine Diphosphate
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Bacterial Proteins
- Catalysis
- DNA Transposable Elements
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Escherichia coli Proteins
- Genes, Bacterial
- Hydrolysis
- Mutagenesis, Insertional
- Mutation
- Phenotype
- Protein Binding
- Recombinant Fusion Proteins
- Thermodynamics
