Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Alternative interactions between the Tn7 transposase and the Tn7 target DNA binding protein regulate target immunity and transposition.

The EMBO journal - 3 Nov 2003

Skelding Zachary, Queen-Baker Jennie, Craig Nancy L

Abstract excerpt

The Tn7 transposon avoids inserting into a target DNA that contains a pre-existing copy of Tn7. This phenomenon, known as 'target immunity', is established when TnsB, a Tn7 transposase subunit, binds to Tn7 sequences in the target DNA and mediates displacement of TnsC, a critical transposase activator, from the DNA. Paradoxically, TnsB-TnsC interactions are also required to promote transposon insertion. We have...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Bacteriophage mu

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA, Bacterial

Share to DNA-Binding Proteins

Share to DNA Transposable Elements

Share to Escherichia coli

Share to Escherichia coli Proteins

Share to Transposases

Source

Open in PubMed Open full text

Identifiers

PMID: 14592987
DOI: 10.1093/emboj/cdg551
PMCID: PMC275408

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Alternative interactions between the Tn7 transposase and the Tn7 target DNA binding protein regulate target immunity and transposition.PMID 14592987Read ↗

Gain-of-function mutations in TnsC, an ATP-dependent transposition protein that activates the bacterial transposon Tn7.Semantic proximity Read ↗

Separate structural and functional domains of Tn4430 transposase contribute to target immunity.Semantic proximity Read ↗

Isolation and characterization of Tn7 transposase gain-of-function mutants: a model for transposase activation.Semantic proximity Read ↗

Analysis of gain-of-function mutants of an ATP-dependent regulator of Tn7 transposition.Semantic proximity Read ↗

Conformational toggling controls target site choice for the heteromeric transposase element Tn7.Semantic proximity Read ↗

Unlocking Tn3-family transposase activity in vitro unveils an asymetric pathway for transposome assembly.Semantic proximity Read ↗

Tn7 recognizes transposition target structures associated with DNA replication using the DNA-binding protein TnsE.Semantic proximity Read ↗

Bacterial transposon Tn7 utilizes two different classes of target sites.Semantic proximity Read ↗

The Tn7 transposase is a heteromeric complex in which DNA breakage and joining activities are distributed between different gene products.Semantic proximity Read ↗

Target DNA bending is an important specificity determinant in target site selection in Tn10 transposition.Semantic proximity Read ↗

The effect of host-encoded nucleoid proteins on transposition: H-NS influences targeting of both IS903 and Tn10.Semantic proximity Read ↗

Enhancement and rescue of target capture in Tn10 transposition by site-specific modifications in target DNA.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.