Article
Increased efficiency of evolved group I intron spliceozymes by decreased side product formation.
RNA (New York, N.Y.) - 1 Aug 2015
Amini Zhaleh N, Müller Ulrich F
Abstract excerpt
The group I intron ribozyme from Tetrahymena was recently reengineered into a trans-splicing variant that is able to remove 100-nt introns from pre-mRNA, analogous to the spliceosome. These spliceozymes were improved in this study by 10 rounds of evolution in Escherichia coli cells. One clone with increased activity in E. coli cells was analyzed in detail. Three of its 10 necessary mutations extended the...
Topics
- Binding Sites
- Introns
- Models, Molecular
- Mutation
- Nucleic Acid Conformation
- RNA Precursors
- RNA Splice Sites
- RNA Splicing
- RNA, Catalytic
- Tetrahymena