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Article

Increased efficiency of evolved group I intron spliceozymes by decreased side product formation.

RNA (New York, N.Y.) - 1 Aug 2015

Amini Zhaleh N, Müller Ulrich F

Abstract excerpt

The group I intron ribozyme from Tetrahymena was recently reengineered into a trans-splicing variant that is able to remove 100-nt introns from pre-mRNA, analogous to the spliceosome. These spliceozymes were improved in this study by 10 rounds of evolution in Escherichia coli cells. One clone with increased activity in E. coli cells was analyzed in detail. Three of its 10 necessary mutations extended the...

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Identifiers

PMID: 26106216
DOI: 10.1261/rna.051888.115
PMCID: PMC4509937

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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Increased efficiency of evolved group I intron spliceozymes by decreased side product formation.PMID 26106216Read ↗

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In vitro genetic analysis of the Tetrahymena self-splicing intron.Semantic proximity Read ↗

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Role of a conserved J8/7 X P4 base-triple in the Tetrahymena ribozyme.Semantic proximity Read ↗

In vivo selection of better self-splicing introns in Escherichia coli: the role of the P1 extension helix of the Tetrahymena intron.Semantic proximity Read ↗

Selection in vitro of an RNA enzyme that specifically cleaves single-stranded DNA.Semantic proximity Read ↗

Two universally conserved adenosines of the group I intron that are important for self-splicing but not for core catalytic activity.Semantic proximity Read ↗

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